Isolation of folate-producing probiotics and regulatory effects on homocysteine metabolism and gut microbiota composition
Isolated folate-producing probiotic strains reduced serum homocysteine and altered gut microbiota in folate-deficient mice.
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What the study showed
The abstract describes screening of over 1,000 bacterial isolates, with selection of folate-producing strains confirmed by whole-genome sequencing and metabolite profiling. In a folate-deficient mouse model, a probiotic cocktail modified serum folate, homocysteine levels, and gut microbiota composition.
How it was done
High-throughput cultivation and screening for probiotic isolation, followed by whole-genome sequencing and metabolic pathway reconstruction. A folate-deficient mouse model was assessed via biochemical assays, qPCR, 16S rRNA sequencing, alpha diversity analysis, PCoA, and LEfSe.
Risk of bias
The abstract is truncated — quantitative results are unavailable, preventing assessment of effect size or statistical significance. Mouse model limits direct extrapolation to humans.
What this study does NOT prove
It cannot be concluded that supplementation with these probiotics corrects folate deficiency or reduces homocysteine in humans.
In clinical practice
No clinical application can be recommended based solely on the abstract of a preclinical study. Translational relevance remains undetermined.
Limitations
The abstract is truncated — quantitative results are unavailable, preventing assessment of effect size or statistical significance. Mouse model limits direct extrapolation to humans.
Technical appendix
Version history
- 1.0 · 2026-09-03 — Auto-generated under Evidence Standard v1.0
Paid access: structured summary from public metadata; consult the original study at the source.