Genistein attenuates ovalbumin-induced food allergy in mice: intestinal barrier, gut microbiota, and metabolites
Genistein pretreatment reduced experimental food allergy signs in mice, with associated changes in gut microbiota and fecal metabolites.
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What the study showed
In an ovalbumin-induced murine food allergy model, genistein administered before and during sensitization attenuated clinical symptom scores, specific antibody responses, mast cell activation, and rectal temperature decline. The treatment was associated with preservation of jejunal tight-junction markers, T-cell modulation, and increased fecal butyrate. Correlation analyses suggested associations between microbial taxa, metabolites, and immune parameters.
How it was done
Murine ovalbumin food allergy model; assessments included immunology, histology, gut microbiome, short-chain fatty acids, and fecal metabolomics. In vitro experiments with bone marrow-derived dendritic cells complemented in vivo findings.
Risk of bias
This is a preclinical mouse study; translation to humans is speculative. The abstract does not detail dose, exact duration, number of animals per group, or controls for genistein's hormonal confounders.
What this study does NOT prove
The study does not demonstrate efficacy, safety, or causal mechanism in humans with food allergy.
In clinical practice
Results do not support any clinical or nutritional recommendation of genistein for food allergy prevention in humans. The preclinical stage requires replication and safety studies before any application.
Limitations
This is a preclinical mouse study; translation to humans is speculative. The abstract does not detail dose, exact duration, number of animals per group, or controls for genistein's hormonal confounders.
Technical appendix
Version history
- 1.0 · 2026-07-27 — Auto-generated under Evidence Standard v1.0
Paid access: structured summary from public metadata; consult the original study at the source.