Amuc_1100 alleviates HFD-induced hepatic lipid accumulation in zebrafish via gut microbiota and intestinal 14-3-3β/α-A protein
The Akkermansia muciniphila outer membrane protein Amuc_1100 reduced high-fat diet-induced hepatic lipid accumulation in zebrafish through gut microbiota modulation and interaction with intestinal protein 14-3-3β/α-A.
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What the study showed
Zebrafish supplemented with 0.01% Amuc_1100 showed lower body weight gain, reduced hepatic lipid accumulation, and decreased microbial translocation compared to the HFD group. Amuc_1100 increased Bacillus abundance and modulated gut microbiota composition. The intestinal protein 14-3-3β/α-A was identified as an Amuc_1100-interacting partner, and its inhibition or knockdown confirmed mechanistic involvement.
How it was done
One-month-old zebrafish were fed LFD, HFD, or HFD plus 0.01% Amuc_1100 for 4 weeks; parallel experiments used larvae, germ-free zebrafish receiving microbiota transplants, and antibiotic-treated zebrafish. Protein interaction was identified by pull-down and co-immunoprecipitation, validated by BV02 inhibitor and gene knockdown.
Risk of bias
The abstract is truncated, precluding access to full validation experiment results and quantitative microbial composition data. The zebrafish model has significant physiological differences from mammals, limiting direct extrapolation to humans or commercially relevant species.
What this study does NOT prove
This study does not prove that Amuc_1100 produces equivalent effects on hepatic lipid accumulation or gut microbiota in humans or mammals.
In clinical practice
No clinical or nutritional recommendation can be derived from this fish animal model study. Mechanistic findings in zebrafish require replication in mammalian models before any translational consideration.
Limitations
The abstract is truncated, precluding access to full validation experiment results and quantitative microbial composition data. The zebrafish model has significant physiological differences from mammals, limiting direct extrapolation to humans or commercially relevant species.
Technical appendix
Version history
- 1.0 · 2026-08-22 — Auto-generated under Evidence Standard v1.0
Paid access: structured summary from public metadata; consult the original study at the source.